Currently Posted:
| Postdoctoral Fellowship:
Plant Secondary Cell Wall (Toulouse, FRANCE) |
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[posted 2 April 2002] |
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| Description: |
A post doctoral fellowship is available starting July 2002 for a maximum
of two years at the CNRS/University Paul Sabatier (UMR 5546) in the team
"Expression and modulation of lignification" headed by Alain M. BOUDET. There is no restriction of citizenship, French and foreign
applicants are equally eligible.
The candidate will be supported by European funds within the framework of the COPOL project : "Integrated control of polysaccharide and lignin
biosynthesis to improve cellulose content and availability and fibre quality". The essential purpose of the programme is the improvement of
raw material quality for the non-food use of plant fibre in pulp and paper industry, textiles, composites for industry, biomass for energy,
through
1.. a better understanding of the biosynthesis of hemicellulosic matrix polysaccharides of the wall to determine their influence on fibre
properties,
2.. the relative influence of the two non-cellulosic components (hemicellulose and lignin) on fibre properties and quality.
Co-ordinate biosynthesis of these two types of non-cellulosic components, synthetised through distinct pathways, will also be studied
through analysis of new lines of genetic material.
The role of the post doc in the next work will be focused on :
a.. the role of laccases, cell wall bound oxidases, on the organization of plant cell wall through the production and the analysis
of transgenic tobacco plants modified for laccase activity
b.. the characterization of proteins bound to the secondary wall of tobacco plants (control and transgenic lines) through a proteomic
approach
c.. the supervision of biochemical analyses in relation with a technician.
Candidates should have an experience in molecular biology genetic engineering and biochemistry.
References :
Chabannes M., Barakate A., Lapierre C., Marita J.M., Ralph J., Danoun S., Halpin C., Grima-Pettenati J., Boudet A.-M. - 2001 - Strong decrease
in lignin content without significant alteration of plant development is
induced by simultaneous down-regulation of cinnamoyl CoA reductase (CR) and cinnamyl alcohol dehydrogenase (CAD) in tobacco plants. Plant
Journal, 28(3), 257-270
Chabannes M., Ruel K., Yoshinaga A., Chabbert B., Jauneau A., Joseleau J.-P., Boudet A.M- 2001 - In situ analysis of lignins from tobacco
transgenic lines down-regulated for several enzymes of monolignol
synthesis reveals a differential impact of transformation on the spatial
patterns of lignin deposition at the cellular and subcellular levels. Plant Journal, 28(3), 271-282
Ranocha Ph., Chabannes M. Danoun S., Jauneau A., Boudet A.M., Goffner D.
- 2002 - Laccase down regulation causes alterations in phenolic metabolism and cell wall structure in poplar. Plant Physiology (sous
presse).
Monthly salary : 1800 euros
Start date : July 1, 2002
Duration : 2 years
Place of work :
Toulouse (France)
Institut Fédératif de Recherche IFR40
"Signalisation Cellulaire et Biotechnologie
végétale"
http://ifr40.smcv.ups-tlse.fr
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| Contact: |
To apply, please send a full CV and a covering letter, including the
names and addresses of two academic referees
Contact person :
Alain M. BOUDET
UMR CNRS-UPS 5546
PÔLE DE BIOTECHNOLOGIE VEGETALE
BP 17 Auzeville
F-31326 CASTANET TOLOSAN
TEL: 33 (0)5 62 19 35 21
FAX: 33 (0)5 62 19 35 02
e-mail: amboudet@smcv.ups-tlse.fr |
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| Postdoctoral Position in Cell Wall Biosynthesis
(Michigan State University) |
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[posted 2 April 2002] |
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| Description: |
A postdoctoral position is available to work on plant cell wall
biosynthesis in the DOE-Plant Research Lab at Michigan State University.
The overall goal is to identify genes and enzymes that synthesize,
regulate, and process the unique hemicelluloses of cereals. The research
will involve one or more of the following projects, depending in part on
the interests of the successful candidate:
(1) proteomics of rice and maize Golgi proteins to identify candidate proteins and genes,
(2) biochemical characterization of glycosyl transferases and glycan synthases,
(3) analysis of natural variation in wall hemicelluloses in maize, rice, and barley using recombinant inbred lines and comparative genomic mapping, and
(4) functional analysis of candidate hemicellulose biosynthetic genes using
RNAi and Agrobacterium-mediated transformation of rice (see Hazen et al.,
Plant Physiol. 128:336, 2002).
This is a unique opportunity to do pioneering work in the fundamental but poorly understood process of cell wall biosynthesis.
Depending on the project, the work will require experience in genome mapping, molecular biology, membrane protein biochemistry, and
proteomics. This research is part of an NSF genomics grant that includes an active
multi-investigator cell wall group within the PRL. Facilities include state-of-the-art mass spectrometric equipment for
proteomics.
The position is available June, 2002, and is initially for one year with the possibility
of renewal.
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| Contact: |
Jonathan Walton, DOE-PRL,
Michigan State University,
E. Lansing, MI 48824
Telephone 517-353-4885;
email: walton@msu.edu.
MSU is an affirmative-action, equal-opportunity employer. |
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