Preparative native gel electrophoresis of digitonin-solubilized cotton plasma membrane proteins. Courtesy, Heungsop Shin and Krystyna Kudlicka. The cotton plasma membrane proteins were solubilized in 0.5 % digitonin and subjected to nondenaturing discontinuous gel electrophoresis to identify and isolate the ß-glucan products synthesized in the gel. All lanes in the above gel were loaded with same sample.

(A) In situ b-glucan synthase activity assay: The gel after electrophoresis was treated with UDP-glucose plus ß-glucan synthase effectors for overnight and the ß-glucan synthase activities were identified in situ by staining the ß-glucan products with calcofluor dye. (The ß-glucan products in the gel were subjected to product analysis by transmission electron microscope)

(B) Coomassie-blue-stain of the same gel.

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