108. Lee, S. Y. and R. M. Brown, Jr. 1987. Experimental cell fusion with selected siphonocladalean algal cells. IN: Cell Fusion. Art Sowers, Ed. Plenum Press, New York, NY. pp 167-178.

108. Introduction

Plant cells frequently have been used for cell-fusion experiments as a result of the development of a technique for enzymatic digestion of the cell wall (Cocking, 1960). With this technique, a variety of new genetic manipulations have become possible, such as protoplast fusion, uptake of organelles, and uptake of DNA. With the enzymatic digestion procedure, large populations of genetically and physiologically homogeneous protoplasts are now available (Chaleff, 1981).The most unique experimental area introduced by the ability to fuse protoplasts is the hybridization of widely divergent taxa that are normally isolated genetically by morphological, developmental, or sexual incompatibility barriers (Salhani et al., 1985). By a method of cell fusion, which involves PEG (polyethylene glycol) and high concentrations of Ca2+, it is possible to construct new and otherwise impossible genetic combinations that can be used for studying the genetic characterization of the plant as well as improving crop quantity and quality.

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Last modified 27 October 2005.
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