122. Brown, Jr. R. M. 1988. Use of Kodak Tri-X pan film and Kodak T-Max 40 professional film in the recording of lattice images of labile specimens with electron microscopy. Tech Bits, Spring 1988, pp. 9-11.

122. Introduction

While the resolving capability of the electron microscope has long been more than adequate to preserve detailed molecular structure for a great number of materials, the major limitation in achieving high resolution electron microscopy of beam sensitive materials has been the lack of a recording medium which will allow both high resolution and low dose. A typical beam-sensitive biological material is cellulose. Cellulose consists of -1, 4-linked glucan polymer chains which are arranged in a crystalline state to form an insoluble submicroscopic rod-like entity known as the microfibril (Figure 1). In 1964, Sugiyamo et al. first succeeded in recording the lattice image of cellulose microfibrils of Valonia macrophysa. Their success was based on the use of a 200 KV electron microscope and a high sensitivity electron micrography film. It was subsequently shown by Revel (1985) that a conventional 120 KV accelerating voltage is adequate for recording lattice images of Valonia cellulose. In the search for a recording medium with sufficient sensitivity and resolution to permit the imaging of the glucan chain lattice of cellulose, we decided to try electron micrography with several standard panchromatic films. Because our Philips 420 electron microscope was equipped with a 35 mm camera port, it was easy to test a number of standard films. To our surprise, we found that both KODAK TRI-X Pan Film and KODAK T-MAX 400 Professional Film provide superior recordings of the glucan chain lattice of cellulose from a variety of sources, in comparison with plate film ordinarily used for electron micrography.

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