130. Saxena, I. M. and R. M. Brown, Jr. 1989. Study of cellulose biosynthesis in Acetobacter xylinum: a genetic approach. In: Cellulose and Wood -Chemistry and Technology, Ed. C. Schuerch. John Wiley and Sons, Inc. N. Y., 537-557.

130. Synopsis

Although a large number of cellulose-synthesizing strains of Acetobacter xylinum harbor plasmid(s), two wild-type strains have been found which do not show the presence of any plasmid DNA. In addition, one plasmid-bearing strain which was completely cured of its plasmid was still capable of synthesizing cellulose. It appears that no correlation exists between the presence of plasmid(s) and the capacity to synthesize cellulose. By screening for altered colony morphology, mutants deficient in pellicle production were isolated. All these mutants produced cellulose II in vivo. Biochemical analysis of these mutants showed normal activity for in vitro cellulose synthesis. Lipopolysaccharide isolated from one of the mutants showed absence of galactose, possibly due to changes in UDPG metabolism. For an analysis of the mutants by complementation, DNA from two strains of A. xylinum was cloned in the broad host-range vectors pRK311 and pMMB33. Recombinant plasmids from A. xylinum ATCC 53582 gene bank constructed in pRK311 were mobilized into the mutants at varying frequencies. A recombinant plasmid that carries a fragment of the plasmid from A. xylinum ATCC 53582 has been identified in the transconjugants in the presence of which the native plasmid is cured. The considerable variability that exists in the biosynthesis of cellulose and its organization in A. Xylinum can now be understood by a detailed genetic analysis of this bacterium.

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Last modified 27 October 2005.
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