137. Lin, F. C., Brown, Jr. R. M., Drake, Jr. R. R., and B. E. Haley. 1990. Identification of the uridine 5'-diphosphoglucose (UDP-glc) binding subunit of cellulose synthase in Acetobacter xylinum using the photoaffinity probe 5-azido UDP-glc. J. Biol. Chem. 265: 4782-4784.

137. Abstract

Photoaffinity labeling of purified cellulose synthase with [-32P]5-azidouridine 5'-diphosphoglucose (UDP-Glc) has been used to identify the UDP-Glc binding subunit of the cellulose synthase from Acetobacter xylinum strain ATCC 53582. The results showed exclusive labeling of an 83-kDa polypeptide. Photoinsertion of [-32P]5-azido-UDP-GIc is stimulated by the cellulose synthase activator, bis-(3'5') cyclic diguanylic acid. Addition of increasing amounts of UDP-Glc pre-vents photolabeling of the 83-kDa polypeptide. The reversible and photocatalyzed binding of this photoprobe also showed saturation kinetics. These studies demonstrate that the 83-kDa polypeptide is the catalytic subunit of the cellulose synthase in A. xylinum strain ATCC 53582.

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Last modified 27 October 2005.
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