An overview of the synthesis and uses of
the photoaffinity analog 5-azido-UDP-glucose in the study of -glucan
synthases from cotton and other systems is presented. When combined
with protein purification techniques and enzymatic activity assays,
photoaffinity labeling with [-32P]5-azido-UDP-glucose
has allowed the identification of the UDP-glucose catalytic subunits
of cellulose synthase from Acetobacter xylinum and Gossypium
hirsutum. An 83 kDa protein was identified as the cellulose
synthase from Acetobacter xylinum preparations. A 37 kDa
protein has been identified as a component of cellulose synthase
from cotton, as well as a 52 kDa protein identified as being involved
in callose biosynthesis. The criteria by which these proteins
were identified and how this type of photoaffinity approach can
be utilized in the study of cellulose biosynthesis are discussed.
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Last modified 21 March 2008.
This document is maintained by R. Malcolm Brown, Jr.