15. Brown, Jr. R. M., Johnson, S. C., and
H. C. Bold. 1968. Electron microscopy and phase contrast microscopy
of sexual reproduction in Chlamydomonas moewusii. J. Phycol.
4:100-125.
15. Summary
The sexual process of C. moewusii
from gametic activation through germination of the zygote has
been studied with phase-contrast and electron microscopy. Long
strands emerging from the gametic flagellar tips are the site
of early flagellar attraction which is followed by union of compatible
flagella within common flagellar sheaths. The gametic connecting
strand is formed by coordinated elongation of the plasma papillae
of a gametic pair and the pene-tration of the former through their
wall papillae while the flagella are in intimate association.
After the free-swimming period, the gametic pairs aggregate in
a second period of clumping. The connecting strand is abscised
and extruded during plasmogamy as are the flagellar basal bodies.
Evidence is presented which suggests union of the gametic plastids,
and stages in karyogamy are illustrated. Formation of the wall
layers, accumulation of starch and lipids, and changes in plastid
organization in the maturing and germinating zygote are described
as is the formation of the gonal walls.