22. Brown, Jr. R. M., Franke, W. W., Kleinig,
H., Falk, H., and P. Sitte. 1969. A cellulosic wall component
produced by the Golgi apparatus. Science 166:894-896.
22. Abstract
The Golgi apparatus of a marine chrysophycean
alga Pleurochrysis scherffelii Pringsheim produces
wall fragments (circular-to-ellipsoidal "scales") which
are released to the periphery by an exocytotic process involving
the fusion of cisternae and the plasma membrane. The cellulosic
component of the scales is a complex network of fibrils (10 to
25 angstroms in diameter) that resist treatment with strong alkali.
Untreated washed scales yield galactose, ribose, arabinose, and
traces of glucose; alkali-purified scales yield much more glucose.
The fibrillar scale constituent shows a positive iodine dichroism
of the intact wall, a positive zinc chloride-iodine reaction,
breakage sites characteristic of highly crystalline cellulose,
and solubility in Schweizer's reagent.