46. McLean, R. J., Laurendi, C. J., and
R. M. Brown, Jr. 1974. The relationship of gamone to the mating
reaction in Chlamydomonas moewusii. Proc. Nat. Acad. Sci.
U.S.A. 71:2610-2613.
46. Abstract
Sexual agglutination of opposite gametes
and agglutination of like gametes by concanavalin A (Con A) were
studied in Chlamydomonas moewusii and C. Reinhardtii
for the possibility that the surface sites involved in these agglutinating
phenomena may be the same. Our data show that the two agglutinating
phenomena appeared at different times after the beginning of gametogenesis.
Sucrose and mannose block agglutination of the gametes by Con
A but do not affect mating ability. Trypsin eliminates mating
ability, except in (-) gametes of C. moewusii, while
Con A agglutinability of gametes by multivalent Con A while mating
ability is unaffected. The data indicate that the mating agglutinin
and the Con A-binding sites are two different flagellar surface
agglutinins that occur coincidentally during gametic differentiation
of both mating types of C. moewusii and C. reinhardtii.
The function of the Con A -binding site on Chlamydomonas gametes
is not known.