47. McLean, R. J. and R. M. Brown, Jr. 1974.
Cell surface differentiation of Chlamydomonas during gametogenesis.
Devel. Biol. 36:279-285.
47. Abstract
Sexual agglutination of opposite
gametes and agglutination of like gametes by concanavalin A (Con
A( were studied in Chlamydomonas moewusii and C. reinhardtii
for the possibility that the surface sites involved in these agglutinating
phenomena may be the same. Our data show that the two agglutinating
phenomena appeared at different times after the beginning of gametogenesis.
Sucrose and mannose block agglutination of the gametes by Con
A but do not affect mating ability. Trypsin eliminates mating
ability, except in (-) gametes of C. moewusii, while Con
A agglutinability remains. Monovalent Con A can selectively bind
to Con A-binding sites to block agglutination of gametes by multivalent
Con A while mating ability is unaffected. The data indicate that
the mating agglutinin and the Con A-binding sites are two different
flagellar surface agglutinins that occur coincidentally during
gametic differentiation of both mating types of C. moewusii
and C. reinhardtii. The function of the Con A-binding
site on Chlamydomonas gametes is not known.