77. Kavookjian, A. M. A. and R. M. Brown,
Jr. 1979. Antigenic properties of the Golgi derived scales of
Pleurochrysis scherffelii. Cytobios 23:45-70.
77. Abstract
The cell wall of the marine alga Pleurochrysis
scherffelii is composed of Golgi-derived scales with four
distinct subcomponents: amorphous coating material, radial microfibrils,
spiral coating substances and spiral microfibrils. The antigenic
properties of the Pleurochrysis scales were studied. Antibodies
were produced in rabbits to the amorphous coating material and
to a heretofore undescribed peripheral subcomponent, known as
the amorphous rim modification substance. The antigenic subcomponents
were detected by agglutination /precipitation, negative staining,
and shadowing procedures. Scales of the Cricosphaera phase
produced negative results when tested with Pleurochrysis scale
antisera. A possible function of the amorphous rim modification
substance in maintaining the integrity of spiral microfibrillar
organization is introduced.