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12. Brown, Jr. R. M. and P. L. Walne. 1967.
Comparative immunology of selected wild types, varieties, and
mutants of Chlamydomonas. J. Protozool. 14:365-373.
12. Synopsis
Rabbit antisera to strains of different
mating types of Chlamydomonas moewusii and to one strain
of C. eugametos were tested against strains of C. reinhardti,
C. eugametos, and C. moewusii, and against strains
of 2 varieties and 6 mutant types of C. moewusii.
Technics of double diffusion, absorption, and immunoelectrophoresis
revealed marked serological differences between the sexually incompatible,
distinct genetic species C. moewusii and C. reinhardti.
Less distinct serological differences were resolved between C.
moewusii and the so-called "species" C. eugametos,
which is sexually compatible with the former, thus reconfirming
the conspecificity between the 2 strains as suggested by Gowans.
Marked serological differences were noted between C. moewusii
and 2 of its varieties (C. moewusii var. tenuichloris
and C. moewusii var. rotunda) which constitute
2 additional genetic species because of sexual incompatibility
between themselves and with C. moewusii. Wild types and
certain mutants of C. moewusii were compared serologically
and could be distinguished on this basis. Strains of different
mating types as well as certain mutant strains (e.g., paralyzed
flagella, flagella-less, twins, and monsters) could be differentiated
serologically although differences were often very subtle. Some
antigens were common to organisms tested.