9. Smith, K. M., Brown, Jr. R. M., Goldstein,
D., and P. L. Walne. 1966. Culture methods for the blue-green
alga Plectonema boryanum and its virus, with an electron
microscope study of virus-infected cells. Virology 28:580-591.
9. Abstract
The blue-green alga Plectonema boryanum
was cultured on a synthetic liquid medium at 22-25 under fluorescent
light. Cultures after 6 days of growth in 125-ml flasks were
inoculated by the addition of 10 ml of partially purified virus
(BGAV). Virus in quantities for purification was obtained from
12-20-1 batches 4 days after inoculation. After infection by
BGAV, photosynthetic lamellae invaginated. Virus particles were
seen in contact with the cell wall and, inside the cell, attached
to photosynthetic lamellae. Here they remained attached after
the cells were completely lysed. Fluorescent and Feulgen staining
indicated more DNA in infected than in healthy cells. Previous
findings that the virus is an icosahedron with a short tail were
confirmed.